Evaluation of spp . , Trichoderma Pseudomonas and for biological control of

spp. is a major pathogenic microbe for tomato, which Background: Ralstonia invades the roots of diverse plant hosts and colonizes xylem vessels causing wilt, especially in tropical, subtropical and warm-temperate regions. Ralstonia spp. produces several virulence factors helping it to invade the plant’s natural defense mechanism. Native isolates of Trichoderma spp., Pseudomonas and can be used as biocontrol agents to control the fluorescens Bacillus subtilis bacterial wilt and combined application of these beneficial microbes can give better results. Bacterial wilt infection in the field was identified by field experts and Methods: the infected plant part was used to isolate spp. in CPG media and Ralstonia was positively identified. Subsequently, the efficacy of the biocontrol agents was tested and documented using agar well diffusion technique and digital microscopy. 2ml of the microbial concentrate (10 cells/ml) was mixed in one liter of water and was applied in the plant root at the rate of 100 ml per plant as a treatment method. It was observed that the isolated spp. AA2 and Results: Trichoderma PFS were most potent in inhibiting the growth of Pseudomonas fluorescens spp. showing ZOI 20.67 mm and 22.33 mm, respectively. Digital Ralstonia , microscopy showed distinct inhibitory effect on the growth and survival of spp The results from the field data indicated that spp. Ralstonia . Trichoderma and alone were able to prevent 92% and 96% of the Pseudomonas fluorescens infection and combination of both were more effective, preventing 97% of infection. Chemical control methods prevented 94% of infection. Bacillus could only prevent 84 % of the infection. subtilis Antagonistic effect against shown by native Conclusions: Ralstonia spp. isolates of spp. and manifested the promising Trichoderma P. fluorescens potential as biocontrol agents. Combined application gave better results. Results shown by were not significant. Bacillus subtilis 1 2